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Follicular development, morphological integrity, and oxidative stress in bovine preantral follicles cultured in vitro with ascorbic acid

Published online by Cambridge University Press:  02 December 2021

Larissa Zamparone Bergamo Open the ORCID record for Larissa Zamparone Bergamo [Opens in a new window] ,
Denis Vinicius Bonato ,
Camila Bizarro-Silva Open the ORCID record for Camila Bizarro-Silva [Opens in a new window] ,
Francieli Gesleine Capote Bonato ,
Tamires Korchovei Sanches ,
Marcela Bortoletto Cerezetti ,
Ana Carolina Rossaneis ,
Waldiceu A. Verri Jr ,
Fábio Morotti  and
Marcelo Marcondes Seneda
Larissa Zamparone Bergamo*
Affiliation:
Laboratory of Biotechnology of Animal Reproduction; Department of Veterinary Clinics; Center of Agrarian Sciences; State University of Londrina; Londrina, Parana, Brazil Department of Veterinary Sciences, Federal University of Parana, Palotina, Parana, Brazil
Denis Vinicius Bonato
Affiliation:
Laboratory of Biotechnology of Animal Reproduction; Department of Veterinary Clinics; Center of Agrarian Sciences; State University of Londrina; Londrina, Parana, Brazil Paranaense University, Umuarama, Parana, Brazil
Camila Bizarro-Silva
Affiliation:
Laboratory of Biotechnology of Animal Reproduction; Department of Veterinary Clinics; Center of Agrarian Sciences; State University of Londrina; Londrina, Parana, Brazil Animal Reproduction Laboratory, School of Life Sciences; Pontifical Catholic University of Parana; Toledo, Parana, Brazil
Francieli Gesleine Capote Bonato
Affiliation:
Laboratory of Biotechnology of Animal Reproduction; Department of Veterinary Clinics; Center of Agrarian Sciences; State University of Londrina; Londrina, Parana, Brazil
Tamires Korchovei Sanches
Affiliation:
Laboratory of Biotechnology of Animal Reproduction; Department of Veterinary Clinics; Center of Agrarian Sciences; State University of Londrina; Londrina, Parana, Brazil
Marcela Bortoletto Cerezetti
Affiliation:
Laboratory of Biotechnology of Animal Reproduction; Department of Veterinary Clinics; Center of Agrarian Sciences; State University of Londrina; Londrina, Parana, Brazil
Ana Carolina Rossaneis
Affiliation:
Department of Pathological Sciences; Biological Sciences Center; State University of Londrina; Londrina, Parana, Brazil
Waldiceu A. Verri Jr
Affiliation:
Department of Pathological Sciences; Biological Sciences Center; State University of Londrina; Londrina, Parana, Brazil
Fábio Morotti
Affiliation:
Laboratory of Biotechnology of Animal Reproduction; Department of Veterinary Clinics; Center of Agrarian Sciences; State University of Londrina; Londrina, Parana, Brazil
Marcelo Marcondes Seneda
Affiliation:
Laboratory of Biotechnology of Animal Reproduction; Department of Veterinary Clinics; Center of Agrarian Sciences; State University of Londrina; Londrina, Parana, Brazil
*
Author for correspondence: Larissa Zamparone Bergamo. Laboratory of Biotechnology of Animal Reproduction; Department of Veterinary Clinics; Center of Agrarian Sciences; State University of Londrina; Londrina, Parana, Brazil. Tel: +55 43 99915 6521. E-mail: larissabergamo1@hotmail.com
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Summary

The aim of this study was to evaluate the follicular development, morphological integrity, and oxidative stress of preantral ovarian follicles from Bos taurus indicus females grown in vitro with ascorbic acid. Ovaries (n = 20) from Bos taurus indicus females were collected, fragmented, and were cultured in vitro for 6 or 12 days in minimum essential medium (MEM), or MEM supplemented with 50 or 100 ng/ml ascorbic acid, with an extracellular matrix of agarose gel, in an incubator at 38.5°C; every 2 days, 100% of the culture medium was replaced. The data were analyzed using the chi-squared test and/or Fisher’s exact test. In the event of a significant effect, the proportions were compared using a 2 × 2 proportion test. The oxidative stress analysis data were submitted to analysis of variance followed by the Bonferroni test. Values were considered significant when P ≤ 0.05. The addition of 100 ng/ml of ascorbic acid to the in vitro culture medium of preantral ovarian follicles from bovine females promoted follicular development, was efficient in maintaining morphological integrity, as well as the stability of reactive oxygen species, after 6 days of in vitro culture.

Keywords

Bos taurus indicusFRAPNBTReactive oxygen speciesTBARS
Type
Research Article
Information
Zygote , Volume 30 , Issue 3 , June 2022 , pp. 391 - 397
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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